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Callus Induction from Seeds of Zea mays Var. EV-2097

Author(s): S.M.S. Naqvi | T. Yasmin | H. Rashid | Z. Chaudary | A. Quraishi

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 5;
Issue: 9;
Start page: 956;
Date: 2002;
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Keywords: Zea mays | callus | 2 | 4-D | cereals | seeds disinfection

The present study was conducted on maize, with the aim to work out a suitable surface disinfecting procedure for seeds and to know the best concentration of 2,4-dichlorophenoxyacetic acid (2,4 -D) for callus induction in N6 medium using var. EV-2097. Better disinfection of seeds was achieved with 30 minutes Clorox treatment (86 %). Maximum callus induction (47 %) was recorded with 2 mg l-1 followed by 3 mg l-1 (18 %) and 1 mg l-1 2,4 -D (16 %).
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