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Callus Induction and Regeneration of Indigenous Garlic (Allium sativum L.)

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Author(s): M.A Salam | 1M.R Ali | Md Eunus Ali | K.A Alam | M.S.H Reza | S Islam | S Islam | S.M.M Rahman

Journal: American Journal of Plant Physiology
ISSN 1557-4539

Volume: 3;
Issue: 1;
Start page: 33;
Date: 2008;
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Keywords: Callus | regeneration | MS medium | 2 | 4-D | BAP

ABSTRACT
The experiment was conducted at plant Biotechnology Laboratory of Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh to study the regeneration of garlic at various combinations and concentrations of growth regulators and also to develop an efficient protocol for regeneration of garlic via callus culture. Vigorously growing leaf discs of garlic strains were used as explants. The explants were collected from cloves germinated in a MS basal medium. Different combinations and concentrations of growth regulators like 2, 4-D, NAA and BA were used in MS medium to observe the callus induction, proliferation and organ formation. The highest callusing was recorded at the best concentration of 2, 4-D (1.0 mg L-1) in MS medium (71.42%). Highest regeneration and highest number of shoots per callus were found in combinations and concentrations of 1.0 mg L-1 NAA plus 1.0 mg L-1 BA (71.42%).
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