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CDNA library from the Latex of Hevea brasiliensis

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Author(s): Wilaiwan Chotigeat | Sarapee Duangchu | Amornrat Phongdara

Journal: Songklanakarin Journal of Science and Technology
ISSN 0125-3395

Volume: 32;
Issue: 6;
Start page: 555;
Date: 2010;
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Keywords: cDNA library | expressed sequence tag | Hevea brasiliensis | latex | rubber tree

ABSTRACT
Latex from Hevea brasiliensis contains 30-50% (w/w) of natural rubber (cis-1,4-polyisoprene), the important rawmaterial for many rubber industries. We have constructed a cDNA library from the latex of H. brasiliensis to investigate theexpressed genes and molecular events in the latex. We analyzed 412 expressed sequence tags (ESTs). More than 90% of theEST clones showed homology to previously described sequences in public databases. Functional classification of the ESTsshowed that the largest category were proteins of unknown function (30.1%), 11.4% of ESTs encoded for rubber synthesisrelatedproteins (RS) and 8.5% for defense or stress related proteins (DS). Those with no significant homology to knownsequences (NSH) accounted for 8.7%, primary metabolism (PM) and gene expression and RNA metabolism were 7.8% and6.6%, respectively. Other categories included, protein synthesis-related proteins (6.6%), chromatin and DNA metabolism(CDM 3.9%), energy metabolism (EM 3.4%), cellular transport (CT 3.2%), cell structure (CS 3.2%), signal transduction (ST2.2%), secondary metabolism (SM 1.7%), protein fate (PF 2.2%), and reproductive proteins (RP 0.7%).
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