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Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells

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Author(s): MIRA Đ. MILISAVLJEVIĆ | GORDANA S. TIMOTIJEVIĆ | DRAGANA B. NIKOLIĆ | JELENA T. SAMARDŽIĆ | VESNA R. MAKSIMOVIĆ

Journal: Journal of the Serbian Chemical Society
ISSN 0352-5139

Volume: 76;
Issue: 9;
Start page: 1229;
Date: 2011;
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Keywords: aspartic proteinase | buckwheat | BY-2 cells | cell wall

ABSTRACT
The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts showed extracellular localization of rFeAPL1-His6 in the cell wall. Based on the obtained results, potential functions of FeAPL1 are discussed.
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