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Chaga mushroom (Inonotus obliquus) induces G/G arrest and apoptosis in human hepatoma HepG2 cells

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Author(s): Myung-Ja Youn, Jin-Kyung Kim, Seong-Yeol Park, Yunha Kim, Se-Jin Kim, Jin Seok Lee, Kyu Yun Chai, Hye-Jung Kim, Ming-Xun Cui, Hong Seob So, Ki-Young Kim, Raekil Park

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 14;
Issue: 4;
Start page: 511;
Date: 2008;
Original page

Keywords: Inonotus obliquus | Cell cycle | G0/G1 arrest | Apoptosis

ABSTRACT
AIM: To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines, HepG2 and Hep3B cells.METHODS: The cytotoxicity of Chaga extract was screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, flow cytometry analysis, Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract.RESULTS: HepG2 cells were more sensitive to Chaga extract than Hep3B cells, as demonstrated by markedly reduced cell viability. Chaga extract inhibited the cell growth in a dose-dependent manner, which was accompanied with G0/G1-phase arrest and apoptotic cell death. In addition, G0/G1 arrest in the cell cycle was closely associated with down-regulation of p53, pRb, p27, cyclins D1, D2, E, cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 expression.CONCLUSION: Chaga mushroom may provide a new therapeutic option, as a potential anticancer agent, in the treatment of hepatoma.
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