Author(s): B.Valentin Bhimba*, Navaneetha Nath and Priyanka Sinha
Journal: Journal of Pharmacy Research
ISSN 0974-6943
Volume: 4;
Issue: 2;
Start page: 477;
Date: 2011;
Original page
Keywords: Silver Nanoparticle | Marine mangrove | Hypocrea lixii | antibacterial analysis
ABSTRACT
Development of reliable processes for the synthesis of silver nanoparticles is an important aspect in Nanotechnology today. Microorganisms like bacteria and fungi have the ability to synthesize metallic nanoparticles. Biosynthetic methods are being developed which serves as an alternative to chemical and physical ones.Biosynthetic methods can be divided into two categories depending upon the location of nanoparticle synthesis i.e. either intracellular or extracellular. In the present study six fungal strains were isolated from marine mangrove sediment from parangipettai on aborauds agar. The pure culture was grown in Saborauds broth at 270 c for 5 days in a shaker incubator. After incubation, the mycelium was separated and washed twice with milli Q water. 20 g of biomass was treated with 200 ml of milliQ water for 72 hours at 270 c and agitated in the shaker incubator. After incubation, the cell was filtered through Whatmann filter paper and the 1mM silver nitrate solution was mixed with 50 ml of cell filtrate and kept at 250c in dark. Control was prepared without addition of Silver nitrate solution. The nanoparticles synthesized showed an absorption peak at 420nm in UV-Vis spectrum corresponding to the Plasmon resonance of silver nanoparticles, thus, confirming their presence. The Fourier transform infrared spectroscopy confirmed the presence of protein as stabilizing agent surrounding the silver nanoparticles. This elite fungal strain was identified and confirmed as Hypocrea lixii by isolating their genomic DNA and amplifying the ITS region present in 5.8s rRNA and sequenced using ITS primer1 and 4.
Journal: Journal of Pharmacy Research
ISSN 0974-6943
Volume: 4;
Issue: 2;
Start page: 477;
Date: 2011;
Original page
Keywords: Silver Nanoparticle | Marine mangrove | Hypocrea lixii | antibacterial analysis
ABSTRACT
Development of reliable processes for the synthesis of silver nanoparticles is an important aspect in Nanotechnology today. Microorganisms like bacteria and fungi have the ability to synthesize metallic nanoparticles. Biosynthetic methods are being developed which serves as an alternative to chemical and physical ones.Biosynthetic methods can be divided into two categories depending upon the location of nanoparticle synthesis i.e. either intracellular or extracellular. In the present study six fungal strains were isolated from marine mangrove sediment from parangipettai on aborauds agar. The pure culture was grown in Saborauds broth at 270 c for 5 days in a shaker incubator. After incubation, the mycelium was separated and washed twice with milli Q water. 20 g of biomass was treated with 200 ml of milliQ water for 72 hours at 270 c and agitated in the shaker incubator. After incubation, the cell was filtered through Whatmann filter paper and the 1mM silver nitrate solution was mixed with 50 ml of cell filtrate and kept at 250c in dark. Control was prepared without addition of Silver nitrate solution. The nanoparticles synthesized showed an absorption peak at 420nm in UV-Vis spectrum corresponding to the Plasmon resonance of silver nanoparticles, thus, confirming their presence. The Fourier transform infrared spectroscopy confirmed the presence of protein as stabilizing agent surrounding the silver nanoparticles. This elite fungal strain was identified and confirmed as Hypocrea lixii by isolating their genomic DNA and amplifying the ITS region present in 5.8s rRNA and sequenced using ITS primer1 and 4.
