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Characterization and antimicrobial activity of natural isolate Lactococcus lactis subsp. lactis BGSM1-19

Author(s): Strahinić Ivana | Cvetanović D. | Kojić M. | Fira Đ. | Tolinački Maja | Topisirović Lj.

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 57;
Issue: 5-6;
Start page: 509;
Date: 2007;
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Keywords: Lactococcus | bacteriocin | antimicrobial activity | Staphylococcus aureus | Micrococcus flavus

The strain Lactococcus lactis subsp. lactis BGSM1-19, isolated from traditionally homemade white cheese, produces two bacteriocins: lactococcin B-like bacteriocin named bacteriocin BacSMa and bacteriocin BacSMb which have shown similarity with lacticin RM. Plasmid curing resulted in a low yield (0.33%) of BacSMa- BacSMas and BacSMa- BacSMas, BacSMb-, BacSMbs derivatives. The bacteriocin biosynthesis was observed in the logarithmic phase of growth and the production plateau was reached after 8 h of incubation at 30oC, when the culture entered the early stationary phase. Biochemical characterization showed that strain BGSM1-19 retained antimicrobial activity within the pH range of 1 to 12 or after treatment at 100oC for 15 min. However, bacteriocin activity was completely lost after treatment with different proteolytic enzymes. The strain BGSM1-19 contains five plasmids. Plasmid curing indicated that genes coding for bacteriocins synthesis and immunity seem to be located on plasmids. BGSM1-19 exhibited antimicrobial activity against some pathogenic bacteria such as Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa and Staphylococcus aureus.
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