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Characterization of Antioxidant activity of Streptomyces species VITTK3 isolated from Puducherry Coast, India

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Author(s): Krishnan Kannabiran

Journal: Journal of Advanced Scientific Research
ISSN 0976-9595

Volume: 1;
Issue: 2;
Start page: 46;
Date: 2010;
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Keywords: DPPH | Streptomyces | Phylogenetic tree | Antioxidant activity.

ABSTRACT
The aim of the present study was to evaluate antioxidant activity of intracellular and extracellular metabolites of Streptomyces species isolated from marine soil sample collected at the Bay of Bengal coast of Puducherry, India. The sample was serially diluted and plated on Starch casein agar and the white powdery colonies formed indicated the growth of actinomycetes and selectively isolated by plated on ISP 1 medium. The broth culture of the same media was used for fermentation process and intracellular and extracellular metabolites were extracted using solvents acetone and ethyl acetate. The brown colored extract obtained was dissolved in water and screened for DPPH radical scavenging activity. The extracellular metabolite showed 96% inhibition at 5mg/ ml, however intracellular metabolites showed only 22% of inhibition at 5 mg/ ml of intracellular metabolites and the inhibition was compared with the standard antioxidant ascorbic acid which showed 97% inhibition at 5mg/ ml concentration. The potential strain was characterized by polyphasic molecular taxonomic approach. The strain was identified as Streptomyces species and designated as Streptomyces sp. VITTK3. The 16 S rDNA sequence was submitted to the Genbank under the accession number GU808333. The phylogenetic tree by neighbor joining Kimura method showed 97% similarity with Streptomyces cebimarensis with the bootstrap value of 20. Secondary structure and the restriction sites were predicted for the strain Streptomyces sp. VITTK3.
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