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Characterization of the dUTpase- Integrase Region of the Pol Gene of Small Ruminant Lentiviruses from Greece

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Author(s): Y.M. Eltahir

Journal: International Journal of Animal and Veterinary Advances
ISSN 2041-2894

Volume: 3;
Issue: 3;
Start page: 170;
Date: 2011;
Original page

Keywords: Caprine arthritis encephalitis | maedi visna | phylogeny | pol | small ruminat lentiviruses

ABSTRACT
Despite the wide spread of Small Ruminant’s Lentiviruses (SRLV), Maedi Visna Virus (MVV) and caprine arthritis-encephalitis (CAEV) in Greece, sequence information on the pol gene of Greek SRLV (GrSRLV) is not reported before. The present study was aimed to establish a sequence database in a conserved region of the viral genome which can then serve to perform phylogenetic analysis. Three hundred and seventy six nucleotides (dUTPase- integrase) region of the pol gene of GrSRLV from different mixed flocks were obtained by semi-nested PCR using degenerate primers. GrSRLV showed 79 and 81% homology in their nucleotides and deduced amino acids sequences respectively. Molecular epidemiology based on the pol sequences indicated that GrSRLV contains both type A and B viruses and the occurrence of five genetically different clusters of SRLV circulating in Greece. The results suggest that GrA-SRLV have a different origin from that of British, Icelandic and South African SRLV reference strains. It also gives additional evidence of interspecies transmission of these viruses between sheep and goats.
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