Academic Journals Database
Disseminating quality controlled scientific knowledge

Characterization of the dUTpase- Integrase Region of the Pol Gene of Small Ruminant Lentiviruses from Greece

Author(s): Y.M. Eltahir

Journal: International Journal of Animal and Veterinary Advances
ISSN 2041-2894

Volume: 3;
Issue: 3;
Start page: 170;
Date: 2011;
Original page

Keywords: Caprine arthritis encephalitis | maedi visna | phylogeny | pol | small ruminat lentiviruses

Despite the wide spread of Small Ruminant’s Lentiviruses (SRLV), Maedi Visna Virus (MVV) and caprine arthritis-encephalitis (CAEV) in Greece, sequence information on the pol gene of Greek SRLV (GrSRLV) is not reported before. The present study was aimed to establish a sequence database in a conserved region of the viral genome which can then serve to perform phylogenetic analysis. Three hundred and seventy six nucleotides (dUTPase- integrase) region of the pol gene of GrSRLV from different mixed flocks were obtained by semi-nested PCR using degenerate primers. GrSRLV showed 79 and 81% homology in their nucleotides and deduced amino acids sequences respectively. Molecular epidemiology based on the pol sequences indicated that GrSRLV contains both type A and B viruses and the occurrence of five genetically different clusters of SRLV circulating in Greece. The results suggest that GrA-SRLV have a different origin from that of British, Icelandic and South African SRLV reference strains. It also gives additional evidence of interspecies transmission of these viruses between sheep and goats.
Why do you need a reservation system?      Save time & money - Smart Internet Solutions