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Characterization of Lycopersicon Species Leaves Anti Fungal Compounds Through Improved Thin Layer Chromatographic Techniques

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Author(s): Ali Ayaz Khan | John C. Snyder

Journal: Asian Journal of Plant Sciences
ISSN 1682-3974

Volume: 2;
Issue: 14;
Start page: 1015;
Date: 2003;
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Keywords: Lycopersicon sp | trichome | sesquiterpenes | cladosporium cucumerinum | inoculum | cho omatography

ABSTRACT
Experiments were conducted to characterize the Lycopersicon hirsutum f.typicum three accession LA.1777, LA.1927, LA.1363 and L. esculentum leaf surfaces trichomes exudates and direct leaf disks trichomes, separated on TLC plates in hex/ether (80:20) and tested the anti fungal properties of trichomes against the challenging fungi C.cucumerinum Ell. and Arthur. It was found that reduced level to 5ul crude extract of L.hirsutum LA. 1777 separated on silica gel plate in hex/ether (80:20) showed significantly strong anti fungal activity and inhibitory zone in the third region. While no fungi toxic activity was detected on TLC for accession, LA.1927, LA.1363 and L. esculentum 5ul volume level crude extracts.

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