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Characterization of transcription factors binding to-120 GATA motif of rat βbminy-globin promoter

Author(s): Pavlović Sonja T. | Mitrović Tatjana | Karan-Đurašević Teodora | Nikčević Gordana T.

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 55;
Issue: 5-6;
Start page: 347;
Date: 2005;
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Keywords: rat β-globin genes | transcription regulation | GATA-1

The aim of this study was to elucidate the regulation of rat adult βbminy-globin gene transcription. We used DNasel foot printing, gel mobility shift and super shift assays to characterize transcription factors involved in this regulation. In this study we analyzed GATA motif at-120 bp in the distal promoter of βbminy-globin gene. Footprint analysis revealed the binding of nuclear factors from MEL cells to the GATA motif. By using gel mobility shift assay two protein complexes were detected. The faster migrating complex was erythroid-specific and more abundant in differentiating MEL cells. Competition experiments with GATA-1 oligonucleotides and GATA-1 protein antibodies confirmed binding of GATA-1 transcription factor to GATA motif at - 120 bp regulation of rat adult βbminy-globin gene.
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