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Chromosome numbers, meiotic behavior and pollen fertility in a collection of Paspalum nicorae Parodi accessions

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Author(s): Camila Aparecida de Oliveira dos Reis | Maria Teresa Schifino-Wittmann | Miguel Dall´Agnol

Journal: Crop Breeding and Applied Biotechnology
ISSN 1518-7853

Volume: 8;
Issue: 3;
Start page: 212;
Date: 2008;
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Keywords: apomixis | meiotic behavior | chromosome number | pollen viability | Paspalum nicorae

ABSTRACT
Chromosome number, meiotic behavior and pollen viability were evaluated in a collection of 53 Paspalumnicorae Parodi accessions, which are part of a breeding project of the species. All accessions are tetraploid, with 2n=4x=40.Despite the invariable chromosome numbers, there was variation among accessions in the frequencies of different chromosomeconfigurations at diakinesis and metaphase I, such as univalents, trivalents and quadrivalents. Other abnormalities asbridges and laggards were also observed at anaphase and telophase I. Meiotic indexes ranged from 82.00 to 99.50% andpollen viability from 88.99 to 95.06%. As the species is pseudogamous apomictic, fertile pollen is necessary for endospermformation. Results show that all plants are meiotically stable and have enough fertile pollen to be used as male parents incontrolled crosses.

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Tangokurs Rapperswil-Jona

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