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Classic and alternative methods in the pyrogen assay

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Author(s): Daniel Francisco Arencibia Arrebola | Luis Alfredo Rosario Fernández

Journal: RETEL : Revista de Toxicología en Línea
ISSN 1668-091X

Volume: 24;
Start page: 1;
Date: 2009;
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Keywords: Pyrogen assays | alternative methods | blood total assays

ABSTRACT
In this letter to the editor stand out the use of the in vivo and in vitro (alternatives) methods, for the pyrogenicity evolution of medications, vaccines. Inside the in vivo methods stands out the gold test of the pyrogenicity or call rabbits pyrogen assays and inside the alternative methods stands out the LAL (Limulus Amebocyte Lisate), the human monocites isolated assay, cellular lines of monocites and the blood total assays. Also have been validated the techniques in blood human cytosine 1 (IL-1), blood human cytosine 6 (IL-6), PBMC IL-6 (monocytic cells line of peripheral blood), MM6 IL-6 (human monocytoid cell line MONO MAC 6) and cryopreserved whole blood citocyne 1 (IL-1).
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