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Clonal Propagation of Different Cultivars of Pelargonium graveolens (L’ Herit.) viz., Reunion, Bourbon and Egyptian

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Author(s): Rashmi P. Tembe | Manjushri A. Deodhar

Journal: Biotechnology
ISSN 1682-296X

Volume: 9;
Issue: 4;
Start page: 492;
Date: 2010;
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Keywords: shoot cultures | nodal explants | clonal propagation | Pelargonium graveolens | root induction | hardening

ABSTRACT
Pelargonium graveolens is a highly valued aromatic plant mainly grown for its rose like essential oil called Geranium oil. In India different cultivars of Pelargonium graveolens are cultivated in varied agroclimatic zones. Algerian cultivar is grown in North Indian plains while Bourbon cultivar is grown in hilly regions of Southern India. These varieties are very difficult to differentiate morphologically. But they could be differentiated by Gas chromatography on the basis of relative proportion of Geraniol and Citronellol. Always Geraniol rich varieties are preferred which impart rosy smell to the essential oil. The germplasm collection of Kelkar Education Trust’s Scientific Research Centre has identified a Geraniol rich variety called Reunion which is suitable for commercial cultivation in the plateau region of Maharashtra. A protocol for clonal propagation for Reunion cultivar was established using nodal explant. Requirement of growth regulators for shoot initiation, shoot proliferation and root induction for all the three cultivars viz., Reunion, Bourbon and Egyptian was compared. Further the in vitro raised plantlets were hardened successfully.
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