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Cloning and Characterization of Three B Hordein Genes From Hordeum vulgare ssp. agriocrithon

Author(s): Z.E. Pu | Y.C. Hou | X.X. Xu | Z.H. Yan | Y.M. Wei | X.J. Lan | Y.L. Zheng

Journal: Journal of Biological Sciences
ISSN 1727-3048

Volume: 8;
Issue: 8;
Start page: 1343;
Date: 2008;
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Keywords: Barley | B hordein gene cloning | sequence analysis

Three novel B hordein genes (designated as Ha1, Ha2 and Ha3) were isolated from the genomic DNA of Hordeum vulgare ssp. agriocrithon accession ZYM0834 by PCR amplification. The coding regions of Ha1, Ha2 and Ha3 were 900, 945 and 975 bp, respectively. The deduced amino acid sequences were 297, 312 and 322 amino acid residues each with a signal peptide, a central repetitive region rich in proline and glutamine and C-terminal non-repetitive domains. Seven (for Ha1 and Ha2) and eight (for Ha3) cysteine residues were found in the C-terminal domain. In order to investigate the three novel B hordein genes belong to B1 or B3 subfamily, a comparison was carried out of these genes with other known B1 and B3 type hordein genes and the phylogenetic tree was constructed. The results indicated that Ha2 and Ha3 were most similar to two B3 type hordein genes respectively and belong to the B3 sub-family. The results provide new information on the variability of B hordein genes.
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