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Cloning and Expression Analysis of Goat cAMP-Responsive Element Modulator

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Author(s): Wenjuan Xun | Liguang Shi | Wenbin Yue | Chunxiang Zhang | Youshe Ren

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 11;
Issue: 9;
Start page: 1427;
Date: 2012;
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Keywords: cAMP-responsive element modulator | goat | cloning | expression | spermatid maturation

ABSTRACT
cAMP Responsive Element Modulator (CREM) as a cAMP-responsive member of the bZIP family of transcription factor has been demonstrated to play an important role in male fertility. In the present study, researchers cloned the goat CREM from testis by RT-PCR, 3' and 5' RACE. The full-length cDNA was 1183 bp with a 960 bp Opening Reading Frame (ORF) encoding 319 amino acids which shares 84.7-99.4% similarity with the human, sheep, dog, mouse and cattle. The deduced amino acid sequence contained two Gln-rich regions, a PKA phosphorylation site and a DNA-binding domain. Goat CREM mRNA was detected in all tissues investigated in this study. The highest expression level of CREM mRNA was observed in testis whereas the lowest level was found in lung. The expression level of CREM mRNA in testis was increased with the age. This expression pattern was similar to that in mouse and human. Furthermore, immunohistochemistry study showed CREM mainly expressed in round spermatids but not in spermatogonium and spermatocyte. These results indicated that CREM may play a key role in maintain normal physiological functions and development in goat, especially in spermatid maturation.
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