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Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus

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Author(s): Heather J. Montgomery | Andrea L. Dupont | Hilary E. Leivo | J. Guy Guillemette

Journal: Biochemistry Research International
ISSN 2090-2247

Volume: 2010;
Date: 2010;
Original page

ABSTRACT
The nitric oxide synthase-like protein from Bacillus cereus (bcNOS) has been cloned, expressed, and characterized. This small hemeprotein (356 amino acids in length) has a mass of 43 kDa and forms a dimer. The recombinant protein showed similar spectral shifts to the mammalian NOS proteins and could bind the substrates L-arginine and NG-hydroxy-L-arginine as well as the ligand imidazole. Low levels of activity were recorded for the hydrogen peroxide-dependent oxidation of NG-hydroxy-L-arginine and L-arginine by bcNOS, while a reconstituted system with the rat neuronal NOS reductase domain showed no activity. The recombinant bcNOS protein adds to the complement of bacterial NOS-like proteins that are used for the investigation of the mechanism and function of NO in microorganisms.
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