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Cloud point method applied for the Apolipoprotein A-I extraction from human plasma and its identification by tandem mass spectrometry

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Author(s): Aline Soriano Lopesa | Marcelo Anselmo Oseas da Silva | Fábio Cesar Gozzo | Marco Aurélio Zezzi Arruda

Journal: Journal of Integrated OMICS
ISSN 2182-0287

Volume: 1;
Issue: 1;
Start page: 55;
Date: 2011;
Original page

Keywords: Protein extraction | Apolipoprotein A-I | Gel electrophoresis | Mass spectrometry

ABSTRACT
This work describes the extraction of the Apolipoprotein A-I (ApoA-I) from human plasma using the cloud point extraction (CPE). The CPE was carried out with a nonionic surfactant (5% w/v Triton® X-114), and the presence of a salting-out effect (10% w/v NaCl) promoted biocom-patible separation conditions at room temperature and pH 6.8. The ApoA-I present in the surfactant-rich phase was identified by tandem mass spectrometry after two-dimensional gel electrophoresis.
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