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Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer

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Author(s): Hamideh Monfared | Seyed Amir Mohsen Ziaee | Mahmoud Hashemitabar | Hamid Khayatzadeh | Vahid Kheyrollahi | Mahmood Tavallaei | Seyed Javad Mowla

Journal: Urology Journal
ISSN 1735-1308

Volume: 10;
Issue: 3;
Start page: 981;
Date: 2013;
Original page

Keywords: biological markers | analysis | urinary bladder neoplasms | miR-21 | TGF-β | gene expression profiling

ABSTRACT
Purpose: To investigate a potential alteration in the expression levels of transforminggrowth factor β (TGF-β) and miR-21 in bladder cancer tissues.Material and Methods: Using real-time polymerase chain reaction (PCR) method, weexamined a potential correlated expression of miR-21 and TGF-β variants in 30 bladdertumors and their marginal/non-tumor biopsy specimens obtained from the same patients.Results: Our data revealed a significant down-regulation of TGF-β variants (P = .03) alongwith a non-significant alteration in the expression of miR-21 in tumor vs. non-tumor samples.However, in contrast to low-grade tumors, the expression of miR-21 was upregulatedin high-grade ones, and the expression level can efficiently discriminate low-grade tumorsfrom high-grade ones (P = .03).Conclusion: In accordance to the observed similarity between TGF-β variants and miR-21gene expression alterations in bladder tumors, treating 5637 bladder cancer cell line withTGF-β recombinant protein caused a significant upregulation of miR-21. The later findingfurther confirmed a correlated expression of TGF-β and miR-21 in bladder tumors.
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