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Comparação entre duas técnicas de análise de fibras elásticas pulmonares em coelhos prematuros

Author(s): Fernanda Polastri Thereza | Mariana Mendes Silva | Rodrigo Leonel dos Santos | Bruno Garcia de Aquino | Celso Moura Rebello | Renata Suman Mascaretti | Roberta Munhoz Manzano

Journal: ConScientiae Saúde
ISSN 1677-1028

Volume: 11;
Issue: 2;
Start page: 217;
Date: 2012;
Original page

Introduction: Bronchopulmonary dysplasia is characterized by a blockage in lung development. Objective: To compare the efficiency of two techniques of analysis of elastic fibers in lungs of premature rabbits. Methods: Preterm rabbits born on the 28th and 29th days of gestation were subjected to ambient air or oxygen for 11 days, randomized into four groups, Ar 28 (n=17), O2 28 (n= 7), Ar 29 (n=8), O2 29 (n=8). The lungs were removed and stained for analysis of elastic fibers. The Nikkon E600 microscope and the image analysis software (Image- Pro Plus 4.5r) were used for elastic fiber analysis. To compare the analysis between microscope and program was used paired "t" test. Results: There were significant differences between the proportion of elastic fibers performed on the microscope and at the program (p
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