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Comparación de la tinción fluorescencia modificada y Gram, en muestras urogenitales y perianales de pacientes asistidos en el área de Infecciones de Transmisión Sexual del Ambulatorio Arquímedes Fuentes, Cumaná estado Sucre

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Author(s): Evelin M. Flores F | Luzmila S. Albarado Y | Diannolys E.Thomas B | Aníbal.Lobo

Journal: Salus Online
ISSN 1316-7138

Volume: 12;
Issue: 2;
Start page: 57;
Date: 2008;
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Keywords: gramnegative diplococcus | Gram staining | differential fluorescent staining method | acridine orange | sodium fluorescein

ABSTRACT
The clinical use of differential stains for detecting sexually transmitted infections is most useful as a guide for early therapeutic decisions. Fazii et al. (2002) designed the differential fluorescent staining method with acridine orange, decolorated with alcohol-acetone and sodium fluorescein, to establish the distinction by fluorescence between grampositive (yellow-looking) and gramnegative (greenlooking) bacteria. Based on this, the purpose of this study was to evaluate the modified differential fluorescent staining method against the Gram in the identification of bacteria in urethral, endocervical and perianal specimens of 49 patients from the Department of Sexually Transmitted Diseases at “Dr. Arquimedes Fuentes Serrano” primary care clinic in Cumana, Sucre State, Venezuela. The fluorescent staining method, according to Fazii et al. (2002), was modified adjusting the fluorescein solution to pH 6.5. The modified fluorescent staining method identified a greater number of specimens with bacteria than the Gram method. On the other hand, in the urethral and perianal smears dyed with the modified differential fluorescent staining method, all the bacteria with a diplococcus morphology emitted an orange fluorescence; only in one endocervical smear an orange fluorescence diplococcus was observed; in the rest, a green color with low fluorescence was seen. This can be explained on the basis ARN synthesis during the bacterial cellular cycle. The modified differential fluorescent staining method, probably allows distinguishing by optical microscopy the genetic expression of diplococcus, mainly in terms of the process of ARN transcription during its cellular cycle. Therefore,considering this finding, as well as its importance for diagnosing gonorrhoea, it represents a significant contribution to continue studies that will evaluate by microscopy the staining characteristics with the Gram and fluorescence methods, along with cultures, molecular techniques and electron microscopy, with the propose of providing more data regarding ultrastructure, physiology and pathogen mechanisms of gonococcus and other bacteria.

Tango Jona
Tangokurs Rapperswil-Jona

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