Academic Journals Database
Disseminating quality controlled scientific knowledge

Comparative genomics of Helicobacter pylori isolates recovered from ulcer disease patients in England

ADD TO MY LIST
 
Author(s): Kauser Farhana | Hussain M Abid | Ahmed Irshad | Srinivas Sriramula | Devi S Manjulata | Majeed Ahmed | Rao K Rajender | Khan Aleem | Sechi Leonardo | Ahmed Niyaz

Journal: BMC Microbiology
ISSN 1471-2180

Volume: 5;
Issue: 1;
Start page: 32;
Date: 2005;
Original page

ABSTRACT
Abstract Background Genomic diversity of H. pylori from many different human populations is largely unknown. We compared genomes of 65 H. pylori strains from Nottingham, England. Molecular analysis was carried out to identify rearrangements within and outside the cag-pathogenicity-island (cag PAI) and DNA sequence divergence in candidate genes. Phylogenetic analysis was carried out based on various high-resolution genotyping techniques. Results Analyses of virulence genes (cagT, cagE, cagA, vacA, iceA, oipA and babB) revealed that H. pylori strains from England are genetically distinct from strains obtained from other countries. The toxigenic vacA s1m1 genotype was found to be less common and the plasticity region cluster was found to be disrupted in all the isolates. English isolates showed a predominance of iceA1 alleles and a functional proinflammatory oipA gene. The English H. pylori gene pool revealed several Asian/oriental features. This included the predominance of cagA – glr (cagA right junction) motif types III and II (up to 42%), presence of vacA m1c alleles and phylogenetic affinity towards East Asian / Amerindian gene pools based on fluorescent amplified fragment length polymorphism (FAFLP) analysis and glmM sequence analysis. Conclusion Overall, our results demonstrated genetic affinities of H. pylori in England with both European and the Asian gene pools and some distinctive genetic features of virulence genes that may have evolved in this important European population.

Tango Jona
Tangokurs Rapperswil-Jona

     Affiliate Program