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Comparative Investigation on Antimicrobial Property of Miliusa Tomentosa leaf Oil and leaf Extract

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Author(s): Vishal Bhagwan Badgujar | Sanjay Javerilal Surana

Journal: Iranian Journal of Pharmacology and Therapeutics
ISSN 1735-2657

Volume: 10;
Issue: 1;
Start page: 7;
Date: 2011;
Original page

Keywords: Antimicrobial Activity | Antifungal | Miliusa Tomentosa | Annonaceae Family | Volatile Oil | Aqueous Extract

ABSTRACT
Aqueous extract and volatile oil were obtained from Miliusa tomentosa by using soxhlet extractor and hydrodistillation with a Clevenger-type apparatus respectively. The extract and volatile oil both were screened for Antimicrobial activity against different bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pncumoniae,, Pseudomonas aeruginosa, Bacillus pumilis and fungi Candida albicans. Aspergillus niger, Fusarium monoliforme, Trichoderma viridae, Phanerochaete chrysosporium and Pcilomyces species by cup plate diffusion method. Minimal Inhibitory Concen­tration (MIC) values of aqueous extract and volatile oil obtained were determined using modified cup plate method. The aqueous extract exhibited weak activity against all the bacteria and one fungi Candida albicans compare to it Volatile oil shown strong activity against the tested bacteria Escherichia coli, Bacillus subtilis, Pseudommonas aeruginosa, Klebsiella pneumonia and moderate activity was seen against Staphylococcus aureus and Bacillus pumilis, it also showed strong activity against fungi like Candida albicans and Fusarium monoliforme whereas moderate activity was observed on Aspergillus niger, Trichoderma viridae and the weak activity against the remaining fungi exhibited prominent activity against bacteria like Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and fungi like Aspergillus niger and Trichoderma viridae as compared to aqueous extract of leaves.
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