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Comparative proteomic map among vanA-containing Enterococcus isolated from yellow-legged gulls

Author(s): Hajer Radhouani | Patrícia Poeta | Luís Pinto | Ricardo Monteiro | Júlio Nunes-Miranda | Susana Correia | Selma Vieira | Carlos Carvalho | Jorge Rodrigues | María López | Carmen Torres | Rui Vitorino | Pedro Domingues | Gilberto Igrejas

Journal: Journal of Integrated OMICS
ISSN 2182-0287

Volume: 2;
Issue: 1;
Start page: 46;
Date: 2012;
Original page

Keywords: Antimicrobial resistance | Bioinformatics | Databases | Proteomics | VanA-containing Enterococcus | Virulence factors

The increase of vancomycin resistance enterococci (VRE) represents a threat for patient care and creates a reservoir of mobile resistance genes for other, more virulent pathogens. The existence of VRE in different ecologic niches complicates the understanding of its epidemiology. The aim of the present study was to analyse the proteome of 2 vanA strains recovered from seagull faecal samples. The vanA E. durans and vanA E. faecium strains presented different genomic patterns: tet(M)-tet(L)-erm(B) and tet(M)-tet(L)-erm(B)-hyl, respectively. A total of 123 spots were excised from two-dimensional gel electrophoresis (2-DE) gel of vanA E. durans SG 2 strain, and 16 were successfully identified by using MS, representing 42 different proteins. For the vanA E. faecium SG 50 strain, 93 spots were excised from the 2-DE gel and 23 were identified, representing 47 different proteins. The vancomycin/teicoplanin A-type resistance protein VanA in E. durans SG 2 strain was present in two different spots. The identified proteins have shown diverse functional activities including glycolysis, conjugation, translation, protein biosynthesis, among others. This work reports the impact of proteomics on knowledge of vanA-containing enterococci and will be helpful to further understand the expression of proteins in antibiotic-resistant bacteria.
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