Academic Journals Database
Disseminating quality controlled scientific knowledge

A COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITY OF THE ETHANOLIC EXTRACTS OF PHLOGACANTHUS THYRSIFLORUS, HOTTUYNIA CORDATA, CURCUMA CAESIA AND SYZYGIUM JUMBOS

ADD TO MY LIST
 
Author(s): Ahmed Shagufa | Borah Mukundam | Das Swarnamoni

Journal: International Research Journal of Pharmacy
ISSN 2230-8407

Volume: 3;
Issue: 9;
Start page: 282;
Date: 2012;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: Staphylococcus aureus | Pseudomonas aeruginosa | Escherichia coli | disc-diffusion method.

ABSTRACT
The study was conducted to know and compare the antibacterial activity of the ethanolic extracts of Phlogacanthus thyrsiflorus (EEPT), Hottuynia cordata (EEHC), Curcuma caesia (EECC) and Syzygium jumbos (EESJ) on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa by disc diffusion method. The ethanolic plant extracts were prepared from the bark of Syzygium jumbos and leaves of Phlogacanthus thyrsiflorus, Hottuynia cordata and Curcuma caesia by percolation method. Clinical isolates of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were obtained from the Department of Microbiology, Assam Medical College & Hospital. Disc diffusion method for antimicrobial susceptibility testing was performed according to the standard Kirby Bauer method. The whatmann-1 filter paper discs of 6mm sizes impregnated with the plant extracts were placed on Mueller-Hinton agar plates seeded with bacterial cultures of 0.5 Mc Farland standards. Ciprofloxacin (5μg/disc) was used as positive control. The antibacterial activities were assessed by the presence or absence of inhibition zones after incubating the plates at 370c for 24 hours. The ethanolic extracts of Phlogacanthus thyrsiflorus (EEPT) and Hottuynia cordata (EEHC) showed significant anti-bacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Ethanolic extracts of Syzygium jumbos (EESJ) showed significant activity against Staphylococcus aureus and Escherichia coli while ethanolic extracts of Curcuma caesia (EECC) showed significant anti-bacterial activity against Staphylococcus aureus. Maximum zones of inhibition to Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were exhibited by Phlogacanthus thyrsiflorus, Syzygium jumbos and Phlogacanthus thyrsiflorus respectively.
Affiliate Program      Why do you need a reservation system?