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A comparative study between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of goats in Mosul city

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Author(s): S. H. Arslan | Q. T. Al-Obaidi | M. A. Al-Taliby | S. D. Hassan

Journal: Iraqi Journal of Veterinary Sciences
ISSN 1607-3894

Volume: 24 (Arabic);
Issue: 2;
Start page: 93;
Date: 2010;
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ABSTRACT
This study was conducted to determine seroprevalence of brucellosis in non vaccinated goats in different areas in Mosulcity and to compare Rose Bengal and indirect ELISA tests for the detection of the antibodies. A total of 102 blood serumsamples were examined, representing 396 goats distributed in different areas. Results of Rose Bengal test showed that the totalseroprevalence of brucellosis was (6.8%) and the highest rate in Al-Rahmania (11.1%), and lowest (7.1%) in Al-Muthana area.No seroprevalence of Brucellosis was recorded in Googjaly and Basheka areas. Indirect ELISA test showed that the totalpercentage was (24.5%) and the highest (55%) were reported in Al-Rahmania area and the lowest percentage was (10%) inBasheka area. The compatibility between the two tests was (0.30) on Kappa value indicating sensitivity of indirect ELISA testcompared with Rose Bengal test in detection antibrucella antibodies in serum goats.

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