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A COMPARATIVE STUDY OF HELICOBACTER PYLORI, BIOPSY SAMPLES FROM DIFFERENT GASTRIC LESIONS WITH TRIPLE STAINING AND MODIFIED GIEMSA STAINING

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Author(s): Pavan Kumar Pindi

Journal: International Journal of Bioassays
ISSN 2278-778X

Volume: 2;
Issue: 2;
Start page: 453;
Date: 2013;
Original page

Keywords: Helicobacter pylori | Triple Staining | Giemsa Staining | Gastric Lesions | Biopsy

ABSTRACT
Helicobacter pylori are associated with most of the disorders of gastro-duodenal tract, such as gastritis, gastric ulcers, dyspepsia and gastric carcinoma. Many staining methods have been developed for the detection of these bacteria from gastric biopsies. However most of the methods adopted were shown to be more expensive, less sensitive, time consuming with less background information. In view of this, the present study was carried out to check the validity of triple staining method against modified Giemsa staining. For this study, 60 biopsy specimens were selected from different gastric lesions as chronic gastritis (30), gastric ulcers (10) and gastric carcinoma (20). These specimens were subjected to modified Giemsa staining showed purple to dark purple bacteria with bluish background. The results showed 56.66, 40 and 15 percentage positivity for chronic gastritis, benign gastric ulcers and malignant gastric lesions, respectively. The stained sections of triple staining showed black to dark brown bacteria with pink colored background with 70, 50 and 10 percentage positivity for chronic gastritis, benign gastric ulcers and malignant gastric lesions, respectively. From the present study it is clear that triple staining method is more sensitive than the modified Giemsa staining.
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