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Comparative Study on Bactericidal Effect of Silver Nanoparticles, Synthesized Using Green Technology, in Combination with Antibiotics on Salmonella Typhi

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Author(s): Shweta Rajawat | Mohammad Shums Qureshi

Journal: Journal of Biomaterials and Nanobiotechnology
ISSN 2158-7027

Volume: 03;
Issue: 04;
Start page: 480;
Date: 2012;
Original page

Keywords: Silver Nanoparticles | Salmonella Typhi | Tea Extract | Ampicillin | Gentamicin

ABSTRACT
In this work bactericidal study of silver nanoparticles was taken up in combination with two standard antibiotics, ampicillin and gentamycin, for Salmonella Typhi. The antibacterial activities of antibiotics were increased in the presence of AgNPs against test strains. The higher enhancing effect was observed for ampicillin in comparison to gentamicin against test strains. Silver nanoparticles were synthesized elctrolytically using silver wire of 99% purity as anode and carbon rod wrapped with LDPE as cathode. Silver nitrate [of Merck] of 0.01N is used as an electrolyte. Here tea extract is added as capping and mild reducing agent. The polyphenols theaflavins and thearubigins, present in tea perform the role of stabilizing or capping agents due to their bulky and steric nature. A brown coloured colloidal solution of silver nanoparticles is obtained. The as-synthesized silver nanoparticles were characterized using XRD, TEM and UV-Vis spectroscopy.
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