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Comparision of biofilm production and multiple drug resistance in clinical isolates of Acinetobacter baumanii from a tertiary care hospital in South India

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Author(s): M. Dheepa | Vinitha L Rashme | B. Appalaraju

Journal: International Journal of Pharmacy and Biomedical Sciences
ISSN 0976-5263

Volume: 02;
Issue: 04;
Start page: 103;
Date: 2011;
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Keywords: Acinetobacter baumanii | biofilm detection' multidrug resistance

ABSTRACT
The present work is aimed at to determine biofilm formation in clinical isolates of Acinetobacter baumannii and to determine the antibiotic susceptibility pattern of seventeen different antibiotics and to correlate between biofilm production and multidrug resistance. A total of 50 isolates were screened for biofilm production by both qualitative and quantitative method in Acinetobacter baumannii isolates. Biofilm production is demonstrated with standard tube test method, in which bacterial film lining a culture tube is stained with a cationic dye and visually scaled. In the second microtitre plate method, the optical density of the stained bacterial film is determined spectrophotometrically. In biofilm production, both qualitative (tube method) and quantitative (microtitre plate) method showed 30 isolates (60%) as biofilm producers. Resistance to antibiotics such as ceftazidime, cefepime and pipercillin was comparatively higher among biofilm producers than non-biofilm producers .Our investigations showed a simultaneous emergence of resistance to many antimicrobial agents available and represent a severe threat in the treatment of hospitalized patients. This study demonstrates a high propensity among the clinical isolates of A. baumannii to form biofilm and a significant association of biofilm with multiple drug resistance.
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