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Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis

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Author(s): Shekatkar S | Acharya N | Harish B | Parija S

Journal: Indian Journal of Medical Microbiology
ISSN 0255-0857

Volume: 28;
Issue: 3;
Start page: 238;
Date: 2010;
Original page

Keywords: Leptospirosis | latex agglutination test | microscopic agglutination test | IgM ELISA | diagnosis

ABSTRACT
The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira by culture has a low sensitivity and the microscopic agglutination test (MAT) is time consuming To overcome these problems, a rapid latex agglutination test (LAT) has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis. We compared the efficiency of the LAT to a commercially available IgM ELISA and MAT. A total of 150 serum samples were tested by LAT, IgM ELISA, and MAT. The positivity was 26.7%, 26% and 24% respectively. The sensitivity and specificity of LAT as compared to MAT was 90.62 and 91.96% respectively. Even though LAT and ELISA showed similar results, its rapidity and simplicity made latex agglutination test more suitable as a rapid screening test.
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