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Comparison Of Line Spread Obtained In A Confocal Microscopic Setup With A Conventional Video Microscope

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Author(s): Chakraborty B

Journal: IETE Journal of Research
ISSN 0377-2063

Volume: 53;
Issue: 4;
Start page: 339;
Date: 2007;
Original page

ABSTRACT
Confocal microscopy is a special technique in the field microscopy with wide applications in the areas of surface micro structuring of mental and non-mental surfaces, finger print observations in forensic science, 3d volume rendering of biological specimen etc. The main feature of confocal microscope is higher lateral as well as higher axial resolution. The higher axial resolution of confocal microscope gives rise to optical slicing capability. In the present paper the line-spread function (LSF) is evaluated with the help of edge spread function (ESF) obtained through point scanning of an edge object in reflection mode confocal microscopic setup. The spread of the LSF is also evaluated from the edge image obtained through a conventional transmission mode microscope fitted with a video camera. The comparison of LSF spread yields an estimation of improvement in lateral resolution in a confocal microscopic setup.
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