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Comparison of main models of experimental colitis essential for studies on novel therapies of inflammatory bowel disease

Author(s): Krzysztof Celiński | Tomasz Dworzański | Agnieszka Korolczuk | Maria Słomka | Sebastian Radej | Ryszard Piasecki | Grażyna Czechowska

Journal: Polish Gastroenterology
ISSN 1232-9886

Volume: 17;
Issue: 3;
Start page: 195;
Date: 2010;
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Keywords: experimental colitis | DSS | TNBS

Introduction: Over recent years, a number of studies on complex, however still elusive pathogenesis of inflammatory bowel disease (IBD) has been conducted. In order to perform a more detailed analysis and due to the restricted system of human models, in the ongoing studies scientists use an animal experimental model for colitis. Inflammation of large intestine induced by rectal administration of TNBS or by DSS given in potable water is among most common models of colitis described in available literature. Aim of the study: The aim of this paper was the comparison of two most common experimental models of colitis prosperously applied for determining new methods for control of IBD pathogenesis. Material and methods: The studies were conducted on Wistar rats. In one group of rats inflammation was induced by a single rectal administration of TNBS at the dose of 10 mg diluted in 50% ethanol up to the volume of 0.25 ml. In the other group of rats DSS was administrated in potable water at the concentration of 1.5 % through the period of 7 days. Animals were weighted before and after the experiment. Intestines affected with inflammation were sampled from both groups for histopathological examination. The level of IL-1β, IL-6, IL-10, and TNF-α and MPO was determined in serum and intestinal homogenate using ELISA immune-enzymatic test. Results and conclusions: The model of acute colitis based on the administration of DSS in drinking water was found to cause more intensive inflammation than the TNBS-based model and consequently resulted in a more significant loss of weight in the animals and yielded stronger inflammatory infiltrations. Histopathological examination revealed slightly more extended inflammatory hallmarks with more intensified edema of mucosa and submucosa in the DSS-based model. No difference regarding the levels of cytokines such as: IL-1β, IL-6, IL-10, TNF-α was reported between the two compared models.
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