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A comparison of methods to quantify prolamin contents in cereals

Author(s): Gianluca Giuberti | Antonio Gallo | Francesco Masoero

Journal: Italian Journal of Animal Science
ISSN 1594-4077

Volume: 10;
Issue: 1;
Start page: e2;
Date: 2011;
Original page

Keywords: Extraction Method | Comparison | Prolamin | Cereals

Hydrophobic prolamins are endosperm storage proteins accounting for about 40% of the total protein in most cereals seeds. Despite the absence of a reference method, several procedures have been periodically published to quantify prolamins in cereals. The aim of this study was to compare a conventional fractionation assay (LND) vs three other methods: one based on sequential extractions (HAM) and two rapid turbidimetric procedures (L&H and DRO). Prolamins were extracted in duplicate on barley, corn and wheat samples. For the turbidimetric prolamin evaluation in barley and wheat, a universally available purified gliadin, as alternative to purified zein, was also tested as standard reference material (SRM). The extraction prolamin values were different among grain types (P
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