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Comparison of Neutrophil Apoptosis α-Defensins and Calprotectin in Children with and without Severe Early Childhood Caries

Author(s): Lida Toomarian | Mandana Sattari | Nazanin Hashemi | Nikoo Tadayon | Alireza Akbarzadeh Baghban

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 8;
Issue: 1;
Start page: 11;
Date: 2011;
Original page

Keywords: Severe Early Childhood Caries | α-Defensins | Calprotectin | Neutrophil Apoptosis

Background: The infectious nature of severe early-childhood caries (S-ECC) points to the possible participation of immunologic host responses including neutrophils and their antimicrobial products. Objectives: The aim of this study was to determine the neutro-phil apoptosis, α-defensins (HNP1-3) and calprotectin levels in the saliva of preschool children and the association with S-ECC. Methods: Oral examinations were performed on 87 children aged 3-5 years and non stimulated whole saliva samples were collected. Thirty of these subjects were considered S-ECC children, 30 with moderate caries (MC) and 27 were caries free (CF). To detect apoptosis, cell staining was done with Annexin-V-Fluos and propidium iodide, and they were analyzed by fluorescent microscopy. The concentration of α-defensins and calprotectin were assessed using ELISA. Results: There were no statistical differences between groups considering the HNP1-3 or calpro-tectin salivary levels (p=0.06 and p=0.23, respectively). The HNP1-3 and calprotectin levels were negatively correlated and the correlation was significant in MC group (p=0.03). Lower levels of apoptotic neutrophils were obtained from CF subjects as compared with S-ECC children (p=0.03). Conclusions: Our findings establish that apoptotic mechanisms could be implicated in the immunity responses associated with S-ECC. We cannot yet determine if the level of salivary α-defensins or calprotectin is pre-dictive of S-ECC.

Tango Jona
Tangokurs Rapperswil-Jona

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