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Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis

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Author(s): Hamid Reza Honarmand | Gholamreza Abdollahpour | Seyyed Saeed Eshraghi

Journal: Iranian Journal of Medical Sciences
ISSN 0253-0716

Volume: 35;
Issue: 2;
Start page: 116;
Date: 2010;
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Keywords: Leptospirosis | zoonosis | ELISA

ABSTRACT
AbstractBackground: Leptospirosis is the most common zoonosiswidespread in tropical and temperate countries with low social-economic status. We aimed to compare an ELISA kit withan in-house ELISA assay to test the serum samples of the patientswho were suspicious of leptospirosis according to theirclinical symptoms.Methods: A total of 282 serum samples of patients suspiciousof leptospirosis admitted to hospitals in Rasht city (north ofIran) were examined for sero-diagnosis of leptospirosis. Bloodsamples were obtained with mean time of 6.36 days after theonset of the symptoms. Antibodies were detected using a commercialqualitative and by an in-house semi quantitative IgMand IgG ELISA and the results were compared with microscopicagglutination test (MAT) as the gold standard. Allspecimens with titers ≥320 against a pathogenic serovar inMAT were considered positive for leptospirosis.Results: The results of MAT have demonstrated that 70 serumsamples (24.8%) had a positive reaction with one of the leptospiraserovar. The sensitivity, specificity, positive and negativepredictive values were 87.1% , 91.0 %, 67.8%, and 95.5% for inhouseELISA assay, respectively, and 100%, 42.9%, 36.6%, and100 % for commercial IgM ELISA assay, respectively.Conclusion: Our results showed that IgM ELISA assay is areliable and sensitive method for the laboratory diagnosis ofacute leptospirosis. In-house semi quantitative IgM ELISAwas more specific and commercial IgM ELISA was moresensitive.Iran J Med Sci 2010; 35(2): 116-121.
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