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Comparison of the Variation Intron Sequences of Growth Hormone Gene in Salmo trutta caspius, Salmo salar and Salmo trutta

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Author(s): Abolhasan Rezaei | Sheyda Akhshabi

Journal: Research Journal of Applied Sciences, Engineering and Technology
ISSN 2040-7459

Volume: 4;
Issue: 6;
Start page: 615;
Date: 2012;
Original page

Keywords: Growth hormone gene | Salmo trutta caspius

ABSTRACT
The Salmo trutta caspius are used for studies of the Growth Hormone (GH) gene, this gene have two major functions, first, The studies for genetics polymorphism in Salmo trutta caspius with other salmonids, second, the GH gene cause of increasing metabolism and growth body system in fish, especially salmonids. In this research, we found the full length of the growth hormone gene of Salmo trutta caspius. In related to, first, we isolated DNA genomics from fin tissue for studies about PCR techniques, and then were designed pair of primers from some salmons of GH genes accessed in the Gene Bank. These primers amplified almost, fragments of 810, 310 and 920 bp. The fragments after purification on the gel electrophoresis were sent to the sequencing. In this study we had aim that amount of similarity between Salmo trutta caspius, Salmo salar and Salmo trutta. However the results of morphology are shown that these species had strong similarity with them, but they have lived in the different region. Salmo trutta caspius lives in the Caspian Sea in the north of Iran, but for usually Salmo salar and Salmo trutta, live in the Atlantic Ocean and White Sea. The fragment sequences were compared between Salmo trutta caspius, Salmo salar and Salmo trutta by the GH gene. The results are shown that there is a high homology between Salmo salar and Salmo trutta regarding GH gene.
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