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Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5

Author(s): Bertalan Marcelo | Albano Rodolpho | de Pádua Vânia | Rouws Luc | Rojas Cristian | Hemerly Adriana | Teixeira Kátia | Schwab Stefan | Araujo Jean | Oliveira André | França Leonardo | Magalhães Viviane | Alquéres Sylvia | Cardoso Alexander | Almeida Wellington | Loureiro Marcio | Nogueira Eduardo | Cidade Daniela | Oliveira Denise | Simão Tatiana | Macedo Jacyara | Valadão Ana | Dreschsel Marcela | Freitas Flávia | Vidal Marcia | Guedes Helma | Rodrigues Elisete | Meneses Carlos | Brioso Paulo | Pozzer Luciana | Figueiredo Daniel | Montano Helena | Junior Jadier | de Souza Filho Gonçalo | Martin Quintana Flores Victor | Ferreira Beatriz | Branco Alan | Gonzalez Paula | Guillobel Heloisa | Lemos Melissa | Seibel Luiz | Macedo José | Alves-Ferreira Marcio | Sachetto-Martins Gilberto | Coelho Ana | Santos Eidy | Amaral Gilda | Neves Anna | Pacheco Ana | Carvalho Daniela | Lery Letícia | Bisch Paulo | Rössle Shaila | Ürményi Turán | Rael Pereira Alessandra | Silva Rosane | Rondinelli Edson | von Krüger Wanda | Martins Orlando | Baldani José | Ferreira Paulo

Journal: BMC Genomics
ISSN 1471-2164

Volume: 10;
Issue: 1;
Start page: 450;
Date: 2009;
Original page

Abstract Background Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.

Tango Jona
Tangokurs Rapperswil-Jona

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