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Construction of Lentivirus-Mediated Retinal Pigment Epithelium Cells Stably Expressing Basic Fibroblast Growth Factors

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Author(s): Shi-Wei Huang | Guan-Fang Su | Hong Wu

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 10;
Issue: 9;
Start page: 1227;
Date: 2011;
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Keywords: Retinal pigment epithelium cell | lentivirus | basic fibroblast growth factor

ABSTRACT
The transplantation of Retinal Pigment Epithelium (RPE) cells was a potential therapeutic strategy in some retinal diseases. To construct RPE cells capable of stably and efficiently expressing basic Fibroblast Growth Factors (bFGF) using a lentivirus system, we performed the current study. The genes of bFGF were cloned into a lentivirus expression vector then the recombinant lentivirus was packaged and harvested. RPE cells were infected and the expression of the bFGF was detected by western blot. The results showed that the lentivirus vector can efficiently transfer genes into RPE cells with long-term expression. Therefore, it is likely to culture a great number of human RPE cells in vitro to provide adequate and safe low-cost donor cells for RPE cells transplantation.
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