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CORRELATION BETWEEN PHYTOCHEMICAL SCREENING AND IN VITRO ANTIBACTERIAL ACTIVITY STUDY OF ROSA INDICA LINN. LEAVES

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Author(s): Sahoo Anjulata Manjari | Chakraborti Chandra Kanti | Nayak Sarojini | Kayal Sriti

Journal: International Journal of Research in Ayurveda and Pharmacy
ISSN 2229-3566

Volume: 2;
Issue: 5;
Start page: 1595;
Date: 2011;
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Keywords: Pink Rose | Marron Rose | Ethanolic extract | Methanolic extract | Phytochemical screening | Antibacterial activity.

ABSTRACT
To know the role of different phytochemicals in relation to the in vitro antibacterial activity of both the ethanolic and methanolic extracts of Pink Rose and Maroon Rose varieties of Rosa indica leaves (Family- Rosaceae), present study was conducted. Following standard methods, phytochemical screening of each extract was performed. For antibacterial activity study, both the extracts were used at 1 mg /ml, 5 mg/ml and 20 mg /ml concentrations. From our study it seems that the Pink Rose variety was effective against both Staphylococcus aureus and Escherichia coli. Moreover, the zone of inhibition produced by this variety of rose plant and tetracycline (positive control) was found to be more or less similar against Escherichia coli. On the other hand, both the extracts of the Maroon Rose variety displayed antibacterial activity against Staphylococcus aureus only. From our antibacterial activity study, it is clear that the Pink Rose variety possesses more antibacterial activity than the Maroon Rose variety. Our phytochemical screening and antibacterial activity study indicated that alkaloids, flavonoids, saponins, tannins and phenols were present in both the extracts of the Pink Rose variety. Probably those phytochemicals were responsible for its antibacterial activity. On the other hand, the both the extracts of the Maroon Rose variety seemed to be antibacterially effective due to the presence of flavonoids, saponins, tannins, steroids and phenols.
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