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Covalent Functionalization for Multi-walled Carbon Nanotube (f-MWCNT)-Folic Acid Bound Bioconjugate

Author(s): Jacob M. Ngoy | Sunny E. Iyuke | Wilhelm E. Neuse | Clarence S. Yah

Journal: Journal of Applied Sciences
ISSN 1812-5654

Volume: 11;
Issue: 15;
Start page: 2700;
Date: 2011;
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Keywords: Functionalized multi-walled carbon nanotubes | functionalized carbon nanotubes folic acid | bioconjugates | nanocomposites

In the current concept, covalently functionalized multi-walled carbon nanotube (MWCNT) as a bioconjugate to folic acid (FA); an essential, bioavailable water soluble B-complex vitamin which is usually expressed on the surfaces of most tumoral cells was used. This was rendered possible through the design of a bioreversible binding water-soluble and biocompatible functionalized multi-walled carbon nanotubes (f-MWCNTs). The MWCNT was synthesized through the Chemical Vapor Deposition (CVD). The MWCNTs were covalently functionalized with sulphuric and nitric acids (3:1) at Room Temperature (RT), 50 and 100C to generate the phenol and carboxyl groups. Furthermore, aspartic acid at 230C was used to generate the carboxyl f-MWCNTs groups. The CNTs and f-MWCNTs were both characterized with the aid of a Transmission Electron Microscopy (TEM). The results showed a decreased in mol ratio (COOH/OH) of the f-CNTs from 80 to 20 nm as the temperature increases from RT to 100C. The f-CNTs carboxyl were attached to 3-(N, N-dimethylamino) propylamine (DMP) and FA through 2-(1H-benzotrial-1-yl)-1, 1,3,3-tetramethylurium hexafluorophosphate (HBTU) to generate f-CNTs-FA conjugates. The results of the high resolution nuclear magnetic resonance (H1NMR) and infrared (IR) spectra showed CONH peak shifts bond bioreversible conjugation of FA at 94 and 101.3% and sizes 50 and 170 nm, respectively. The f-MWNT-FA moieties thereby have a greater versatility and can be used for the treatment and restoration of neoplasma cells.
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