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Cryopreservation of Lily [Lilium ledebourii (Baker) Bioss.] Germplasm by Encapsulation-Dehydration

Author(s): B. Kaviani | M.R. Safari-Motlagh | M.N. Padasht-Dehkaei | A.H. Darabi | A. Rafizadeh

Journal: International Journal of Botany
ISSN 1811-9700

Volume: 4;
Issue: 4;
Start page: 491;
Date: 2008;
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Keywords: Cryopreservation | encapsulation | dehydration | germplasm conservation | lily | sucrose

A cryopreservation process using encapsulation-dehydration was set up for seeds of lily [Lilium ledebourii (Baker) Bioss.]. Seeds were subjected to a rapid freezing protocol in liquid nitrogen following encapsulation in MS medium supplemented with 3% Na-alginate, 100 mM CaCl2 and 0.6 M sucrose and then desiccation for 1 h under the laminar air flow. Survival after freezing was nil for control seeds, 22% for seeds treated with sucrose and dehydration and up to 50% for seeds treated with sucrose, dehydration and encapsulation.
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