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Culture media influence on “in vitro” propagation of some sweet and sour cherry rootstocks

Author(s): Catiţa Plopa | Gheorghe Mladin | Valentina Isac | Sergiu Ancu

Journal: Scientific Papers of the Research Institute for Fruit Growing Pitesti, Romania
ISSN 1584-2231

Volume: XXV;
Start page: 189;
Date: 2009;
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Keywords: In vitro | cherry | rootstocks | culture media

Improved in vitro clonal propagation methods are valuable tools for nurseries. The present study reports the trials undertaken to optimise the in vitro clonal propagation ability of three important cherry rootstocks: IPC 2, IPC 3 and IPC 5. Axillary buds explants were grown in axenic culture on defined culture media Murashige & Skoog (MS), Lee & Fossard (LF), Quoirin &Lepoivre (QL) including auxins, giberrellic acid, and cytokinins. Cherry rootstocks responded differently to the same medium and environmental conditions during establishment, proliferation and rooting in vitro. The best results during in all propagation phases was recorded with IPC 3 rootstock. The regenerated shoots of all the three rootstocks species tested grew well on the shoots proliferation medium Lee & Fossard supplemented with 1.0mg l-1 benzyl adenine and 0.2mg l-1 naphthalene acetic acid. Micropropagated shoots of IPC 3 were rooted with good results (73.33 % rooted plants) using 1.5 mg l-1 indolilbutyric acid. In the same conditions the results for IPC 5 was 46.66 % rooted plants and for IPC 2 only 33.33 % rooted plants.
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