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Cytokine Profile in the Endometrium of Normal Fertile and Women with Repeated Implantation Failure

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Author(s): Samira Rajaei | Amir Hassan Zarnani | Mahmood Jeddi-Tehrani | Maryam Tavakoli | Afsaneh Mohammadzadeh | Ali Dabbagh | Mahroo Mirahmadian

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 8;
Issue: 4;
Start page: 201;
Date: 2011;
Original page

Keywords: Cytokine | Endometrium | Implantation | Stromal Cells

ABSTRACT
Background: Repeated Implantation Failure (RIF) is one of the most intricate obstaclesin assisted reproduction. The cytokine and chemokine composition of uterine cavityseem to play important roles in the implantation process. Objective: To compare thecytokine profile in the endometrium of normal fertile women and those with repeatedimplantation failure. Methods: After enzymatic digestion of endometrial tissues, wholeendometrial cells and endometrial stromal cells from RIF and normal fertile womenwere cultivated and stimulated for cytokine secretion. The levels of IL-10, TGF-╬▓,IFN-╬│, IL-6, IL-8 and IL-17 in culture supernatants of the two groups were assayed byELISA and compared together. Results: Endometrial stromal cells and whole endometrialcells of normal fertile women produced higher levels of IL-6, IL-8 and TGF-╬▓compared to RIF group, although this difference was statistically significant only in endometrialstromal cells (p=0.005, 0.002 and 0.001, respectively). In addition, endometrialstromal cells of normal fertile women produced lower levels of IL-10 in comparisonwith RIF group (p=0.005). Conclusion: Disturbances in cytokine production atthe feto-maternal interface could be a cause of implantation failure. A pro-inflammatorycytokine milieu seems to be pivotal for successful implantation.

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Tangokurs Rapperswil-Jona

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