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Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse

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Author(s): Teixeira Mauro M | Williams Timothy J | Hellewell Paul G

Journal: Memórias do Instituto Oswaldo Cruz
ISSN 0074-0276

Volume: 92;
Issue: suppl.2;
Start page: 211;
Date: 1997;
Original page

Keywords: chemokines | eosinophils | eotaxin | recruitment

ABSTRACT
Chemokines (chemoattractant cytokines) induce potent and selective chemotaxis of leukocyte subsets in vitro. Here, we review briefly the chemokines shown to induce eosinophil chemotaxis in vitro and describe a novel model for the study of the ability of chemokines to stimulate eosinophil migration in vivo. Eosinophils were purified from the blood of mice over-expressing the IL-5 gene and labelled with 111In. Only the C-C chemokines, eotaxin and MIP-1alpha, but not RANTES, MCP-1, MCP-3, MCP-4, MIP-1ß, KC and MIP-2, effectively induced the recruitment of 111In-eosinophils in mouse skin. We suggest that this mouse model will be useful in assessing the role of endogenously-generated chemokines in mediating eosinophil migration to sites of allergic inflammation in vivo.
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