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Detection by PCR of Multiple Subgroups of Avian Leukosis Virus (ALV) in Broilers in the Sudan

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Author(s): Maaz Majzoub Abdel-Latif | Abdelmelik Ibrahim Khalafalla

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 4;
Issue: 3;
Start page: 407;
Date: 2005;
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ABSTRACT
An investigation on avian leukosis virus infection in broiler parent farm in Khartoum state, Sudan was conducted. Clinical signs, morbidity rate, mortality rate were recorded. Necropsy was performed, histopathological sections from infected livers were made, and virus isolation trials in chick embryo fibroblast and chorioallantoic membrane were performed. PCR tests were performed on DNA extracted from infected livers and spleen. Affected birds showed in-appetence, abnormal feathering, paleness of combs and wattles, loss of weight, decrease in egg production, depression, paralysis and death. Post mortem examination of affected birds revealed hepatomegaly, spleenomegaly, congestion of the lungs, enlargement of the heart and kidneys. Histopathological sections revealed diffuse infiltration of pleomorphic population of lymphocytes with mitotic figures. Virus isolation attempts failed to yield a virus. PCR tests confirmed that ALV subgroup (A-D), HPRS, and ALV-J were involved in the infection. This was the first report on avian leukosis virus infection in the Sudan.
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