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Detection of Mycobacterium avium subsp. paratuberculosis in asymptomatic bovines by IS900 Polymerase Chain Reaction

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Author(s): Remya Raveendran | Priya P.M. | Koshy John | Krishnan Nair G | Vijayakumar K

Journal: Veterinary World
ISSN 0972-8988

Volume: 4;
Issue: 6.000;
Start page: 248;
Date: 2011;
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Keywords: Paratuberculosis | MAP | IS900 PCR

ABSTRACT
Faecal samples were collected from 58 asymptomatic bovines and after DNA extraction IS900 Polymerase Chain Reaction (IS900 PCR) was performed to detect Mycobacterium avium subsp. paratuberculosis (MAP). Ten samples (17.24 %) were positive for MAP. The results indicated that the IS900 PCR assay can be used for the early diagnosis of bovine paratuberculosis. [Vet. World 2011; 4(6.000): 248-249]

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