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Determination of β-lactam Antibiotics in Pharmaceutical Preparations by Uv-visible Spectrophotometry Atomic Absorption and High Performance Liquid Chromatography

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Author(s): A. J. Abdulghani | H. H. Jasim | A. S. Hassan

Journal: Pakistan Journal of Chemistry
ISSN 2220-2625

Volume: 2;
Issue: 3;
Start page: 150;
Date: 2012;
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Keywords: β-lactam drugs | solvent extraction | complex formation | correlation coefficient | direct methods

ABSTRACT
The determination amoxicillin, ampicillin and cephalexin was studied by complexation of the antibiotics with Au(III) and Hg(II)ions in bulk and pharmaceutical preparations using uv-visible spectrophotometry, atomic absorption, and HPLC techniques.Optimum conditions for complex formation were fixed at pH 4 and (2-4) for Au(III) and Hg(II)complexes respectively, heatingtemperature at (60 °C) and heating time for (10 minute). All complexes were extracted from aqueous solution with benzyl alcoholprior to measurements except in the case of HPLC. The L:M ratios for all complexes were determined and stability constants werecalculated using mole ratio method. The Beer's law was obeyed over the concentration range (5-60) and 5-50 μg/ml of antibioticsfor Au(III) and Hg(II) complexes using colorimetric method and (1-25 μg/ml) of Au(III) for FAAS. The linearity for HPLCmethod was (10-110 and 10-120 μg/ml) respectively. The correlation coefficients (r) were (0.9981-0.9997). Generally, the highestsensitivity was recorded by FAAS.
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