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Determination of Amitraz in the Honey Samples by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography—Flame Ionization Detection

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Author(s): Mostafa Bashiri-Juybari | Ali Mehdinia | Ali Jabbari | Yadollah Yamini

Journal: American Journal of Analytical Chemistry
ISSN 2156-8251

Volume: 02;
Issue: 05;
Start page: 632;
Date: 2011;
Original page

Keywords: Dispersive Liquid–Liquid Microextraction | Amitraz | Honey Sample

ABSTRACT
Dispersive liquid-liquid microextraction (DLLME) followed by gas chromatography–flame ionization detection (GC-FID), as a simple, rapid and efficient method, was developed for the determination of amitraz in honey samples. This method involves the use of an appropriate mixture of the extraction and disperser solvents for the formation of a cloudy solution in 5.0 mL aqueous sample containing amitraz. After extraction, phase separation was performed by centrifugation and the concentrated amitraz in the sedimented phase was determined by gas chromatography—flame ionization detection (GC-FID). Some important parameters such as the type and volume of extraction and disperser solvents, and the effect of pH and salt on the extraction recovery of amitraz were investigated. Under the optimum conditions (13 µL of carbon tetrachloride as an extraction solvent, 1 mL of acetonitrile as a disperser solvent, no salt addition and pH 6) preconcentration factor and the extraction recovery were 955 and 95.5%, respectively. The linear range was 0.01 - 1.0 mg?kg–1 and the limit of detection was 0.0015 mg?kg–1. The relative standard deviation (RSD, n = 4) for 0.1 mg?kg–1 of amitraz was 3.2%. The recoveries of amitraz from honey samples at the spiking levels of 0.1 mg?kg-1 were 78.8 and 98.2%. The results indicated that DLLME is an efficient technique for the extraction of amitraz in honey samples.
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