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Determination of bioaccessibility of phthalate acid esters in food based on an in vitro digestion model

Author(s): LIU Xiao-Yu | LIU Xing | ZHU Yu-Tian | MENG Chao | DAI Yan | XU Shao-Hua

Journal: Shipin Anquan Zhiliang Jiance Xuebao
ISSN 2095-0381

Volume: 4;
Issue: 1;
Start page: 207;
Date: 2013;
Original page

Keywords: phthalate acid esters (PAEs) | bioaccessibility | solid-phase extraction (SPE) | high pressure liuid chromatograpy (HPLC)

Objective An solid-phase extraction-high pressure liuid chromatograpy (SPE-HPLC) method was developed for determination of four kinds of phthalate acid esters(PAEs) in liquid phase and solid phase of digestive juice base on an in vitro digestion model. Methods The food sample was treated via the in vitro digestion model, and the digestive juices were centrifuged to separate supernatant and solid. After extracted using n-hexane and purified by SPE (Florisil column), PAEs in the liquid phase and solid phase were determined by HPLC, respectively, and then the bioaccessibility were calculated. Results The method showed a good linearity over the range of 0.1~20.0 mg/L for PAEs with r=0.998. The recoveries were in range of 70.38%~96.49%, and the relative standard deviations(RSDs) were lower than 7%. Conclusion The proposed method with strong anti-interference capacity is convenient, sensitive and accurate, and it is suitable for the determination of bioaccessibility of PAEs in food, and provides a way for exposure estimation and risk assessment.
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