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Determination of metabolites of nitrofuran in shrimp using ultra pressure liquid chromatography-tandem mass spectrometry

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Author(s): SHAO Bing

Journal: Shipin Anquan Zhiliang Jiance Xuebao
ISSN 2095-0381

Volume: 4;
Issue: 1;
Start page: 135;
Date: 2013;
Original page

Keywords: nitrofuran | metabolite | ultra pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) | shrimp

ABSTRACT
Objective An analytical method for the detection of four metabolites of nitrofuran in shrimp by ultra- perssure liquid chromatography-tandem quadrupole mass spectrometry (UPLC-MS/MS) was established. Methods After derived by 2-NB, the sample extraction was purified by HLB cartridge. The objective compounds were separated using Waters Acquity BEH C18 column with acetonitrile –5 mmol/L ammonium acetate (0.1% acetic acid) as mobile phase and analyzed by UPLC-MS/MS under multiple reaction monitoring mode(MRM). Results The LOQ for four target compounds were 1.0 μg/kg. The average recoveries were 81.6%~95.1% at the level of 1.0, 5.0 μg/kg and 20.0 μg/kg with the relative standard deviation lower than 13.6%. Conclusion The method is suitable for the detection of residual pollutant in shrimp.
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